Crab duplex-specific nuclease
Crab duplex-specific nuclease is a nuclease derived from the red king crab (Paralithodes camtschaticus, kamchatka crab) hepatopancreas that displays a strong preference for cleaving double-stranded DNA and DNA in DNA–RNA hybrid duplexes, compared to single-stranded DNA. Moreover, the cleavage rate of short, perfectly matched DNA duplexes by this enzyme is essentially higher than that for non-perfectly matched duplexes of the same length. It has been applied to SNP detection[1] and RNA normalization.[2]
References
- ^ Shagin, DA; Rebrikov, DV; Kozhemyako, VB; Altshuler, IM; Shcheglov, AS; Zhulidov, PA; Bogdanova, EA; Staroverov, DB; Rasskazov, VA; Lukyanov, S (December 2002). "A novel method for SNP detection using a new duplex-specific nuclease from crab hepatopancreas". Genome Research. 12 (12): 1935–42. doi:10.1101/gr.547002. PMC 187582. PMID 12466298.
- ^ Christodoulou, DC; Gorham, JM; Herman, DS; Seidman, JG (April 2011). "Construction of Normalized RNA-seq Libraries for Next-Generation Sequencing Using the Crab Duplex-Specific Nuclease". Current Protocols in Molecular Biology. Chapter 4: Unit 4.12. doi:10.1002/0471142727.mb0412s94. PMC 3152986. PMID 21472699.
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Hydrolase: esterases (EC 3.1)
ester hydrolases
- Cholinesterase
- Acetylcholinesterase
- Butyrylcholinesterase
- Pectinesterase
- 6-phosphogluconolactonase
- PAF acetylhydrolase
- Lipase
- Bile salt-dependent
- Gastric/Lingual
- Pancreatic
- Lysosomal
- Hormone-sensitive
- Endothelial
- Hepatic
- Lipoprotein
- Monoacylglycerol
- Diacylglycerol
- Alkaline phosphatase
- Acid phosphatase (Prostatic)/Tartrate-resistant acid phosphatase/Purple acid phosphatases
- Nucleotidase
- Glucose 6-phosphatase
- Fructose 1,6-bisphosphatase
- Protein phosphatase
- OCRL
- Pyruvate dehydrogenase phosphatase
- Fructose 6-P,2-kinase:fructose 2,6-bisphosphatase
- PTEN
- Phytase
- Inositol-phosphate phosphatase
- Protein phosphatase: Protein tyrosine phosphatase
- Protein serine/threonine phosphatase
- Dual-specificity phosphatase
Phosphodiesterase
deoxyribonuclease
and ribonuclease)
3.1.11-16: Exonuclease |
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3.1.21-31: Endonuclease |
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